… profile: 95C for 15 s, 50C for 1 h, repeat from step 1, 15×, 4C for at least 10 min… C for 2 min,
95C for 30 s, 65C for 20 s, 72C for 30 s, then repeat from step 2, 11×, 72C for 7min, hold at 4C…

… 96 C for 3 min followed by amplification under the following conditions: denaturation at 96 C
for 30 s, annealing at 57 C for 30 s and elongation at 72 C … elongation step at 72 C for 3 min. …

The parthenogenetic all-female marbled crayfish is a novel research model and potent invader
of freshwater ecosystems. It is a triploid descendant of the sexually reproducing slough …

… c Heatmap showing distinct activations of pathway neurons for the test set samples stratified
by age quantiles. d Pathway ranking based on Pearson correlation coefficients of pathway …

… The sequence amplified in our study (fragment 2) shows substantial sequence similarity to
the C-terminal catalytic domain of known Dnmt1 enzymes. (B) Fragment 2 encodes the highly …

… minimum quality value of the base at C position ≥ 30 and (2) minimum quality value of the
two bases before and after the C position ≥ 20. Only C-positions with a minimum coverage of …

We generated RNA-seq data to assemble the transcriptome of the noble crayfish (Astacus
astacus) from four combined tissues (abdominal muscle, hepatopancreas, ovaries, green …

… We also observed that fibroblast procollagen type I c-peptide production was significantly
(p < 0.0001, RM one-way ANOVA, Dunnett multiple testing correction) increased after …

… Other upregulated genes during aging such as CXCL1 and HLA-C were significantly more
expressed in the MDM than in M1 or M2 macrophages. In contrast, HSPA1B and HSPH1, …

… (C) Boxplot showing the deviation of DNAm from chronological age based on aging phase
outlier status, revealing a divergence in DNAm aging rate for aging phase outliers. Statistical …