[PDF][PDF] Activity-dependent gating of parvalbumin interneuron function by the perineuronal net protein brevican
…, A Marques-Smith, R Deogracias, CM Winterflood… - Neuron, 2017 - cell.com
Activity-dependent neuronal plasticity is a fundamental mechanism through which the nervous
system adapts to sensory experience. Several lines of evidence suggest that parvalbumin …
system adapts to sensory experience. Several lines of evidence suggest that parvalbumin …
Nanoscopic compartmentalization of membrane protein motion at the axon initial segment
D Albrecht, CM Winterflood, M Sadeghi… - Journal of Cell …, 2016 - rupress.org
… The irradiance at the sample was ∼2 kW/cm 2 . The EMCCD camera was run in its high-speed …
A lower irradiance of ∼0.5 kW/cm 2 was used to reduce the extent of photobleaching. …
A lower irradiance of ∼0.5 kW/cm 2 was used to reduce the extent of photobleaching. …
[PDF][PDF] Nanoscale structural plasticity of the active zone matrix modulates presynaptic function
The active zone (AZ) matrix of presynaptic terminals coordinates the recruitment of voltage-gated
calcium channels (VGCCs) and synaptic vesicles to orchestrate neurotransmitter …
calcium channels (VGCCs) and synaptic vesicles to orchestrate neurotransmitter …
[HTML][HTML] Single-molecule microscopy of molecules tagged with GFP or RFP derivatives in mammalian cells using nanobody binders
E Platonova, CM Winterflood, A Junemann, D Albrecht… - Methods, 2015 - Elsevier
… ∼2 kW/cm 2 and the 473 nm activation laser intensity is automatically adjusted to keep the
average number of localizations per frame constant (maximum intensity ∼0.5 kW/cm 2 ). We …
average number of localizations per frame constant (maximum intensity ∼0.5 kW/cm 2 ). We …
[PDF][PDF] Dual-color 3D superresolution microscopy by combined spectral-demixing and biplane imaging
CM Winterflood, E Platonova, D Albrecht, H Ewers - Biophysical journal, 2015 - cell.com
Multicolor three-dimensional (3D) superresolution techniques allow important insight into
the relative organization of cellular structures. While a number of innovative solutions have …
the relative organization of cellular structures. While a number of innovative solutions have …
Nanometer axial resolution by three-dimensional supercritical angle fluorescence microscopy
CM Winterflood, T Ruckstuhl, D Verdes, S Seeger - Physical review letters, 2010 - APS
We report a noninvasive fluorescence microscopy method and demonstrate nanometer
resolution along the optical axis. The technique is based on the influence of the microscope slide …
resolution along the optical axis. The technique is based on the influence of the microscope slide …
A simple method for GFP-and RFP-based dual color single-molecule localization microscopy
E Platonova, CM Winterflood, H Ewers - ACS chemical biology, 2015 - ACS Publications
The recent development of single-molecule localization-based super-resolution techniques
has afforded a resolution in the nanometer range in light microscopy. The ability to resolve …
has afforded a resolution in the nanometer range in light microscopy. The ability to resolve …
Absolute arrangement of subunits in cytoskeletal septin filaments in cells measured by fluorescence microscopy
C Kaplan, B Jing, CM Winterflood, AA Bridges… - Nano …, 2015 - ACS Publications
We resolved the organization of subunits in cytoskeletal polymers in cells by light microscopy.
Septin GTPases form linear complexes of about 32 nm length that polymerize into …
Septin GTPases form linear complexes of about 32 nm length that polymerize into …
Dual color single particle tracking via nanobodies
D Albrecht, CM Winterflood… - Methods and applications …, 2015 - iopscience.iop.org
Single particle tracking is a powerful tool to investigate the function of biological molecules
by following their motion in space. However, the simultaneous tracking of two different …
by following their motion in space. However, the simultaneous tracking of two different …
Single‐molecule localization microscopy using mCherry
CM Winterflood, H Ewers - ChemPhysChem, 2014 - Wiley Online Library
… The imaging laser intensity of the 643 nm laser line used for Alexa Fluor 647 was ∼1.5
kW cm −2 and the intensity for the 556 nm laser line for mCherry was ∼2.0 kW cm −2 . The …
kW cm −2 and the intensity for the 556 nm laser line for mCherry was ∼2.0 kW cm −2 . The …