Despite advances in cancer biology and therapeutics, drug resistance remains problematic.
Resistance is often multifactorial, heterogeneous, and prone to undersampling. Nonetheless…

Large panels of comprehensively characterized human cancer models, including the Cancer
Cell Line Encyclopedia (CCLE), have provided a rigorous framework with which to study …

Oncogenic mutations in the serine/threonine kinase B-RAF (also known as BRAF) are found
in 50–70% of malignant melanomas 1 . Pre-clinical studies have demonstrated that the B-…

To explore the distinct genotypic and phenotypic states of melanoma tumors, we applied
single-cell RNA sequencing (RNA-seq) to 4645 single cells isolated from 19 patients, profiling …

A detailed understanding of the mechanisms by which tumors acquire resistance to targeted
anticancer agents should speed the development of treatment strategies with lasting clinical …

Most patients with BRAF V600 -mutant metastatic melanoma develop resistance to selective
RAF kinase inhibitors. The spectrum of clinical genetic resistance mechanisms to RAF …

Whole-exome sequencing of cell-free DNA (cfDNA) could enable comprehensive profiling
of tumors from blood but the genome-wide concordance between cfDNA and tumor biopsies …

… MPNSTs were seeded at 6.5 × 10 4 cells per 6-cm well in normal growth medium containing
rapamycin (0.01, 0.1, 1.0, 10, or 100 nM) or an equal volume of vector (DMSO). After 7 days, …

Oncogene-induced senescence functions to limit tumor development. However, a complete
understanding of the signals that trigger this type of senescence is currently lacking. We …

Functional characterization of the human genome requires tools for systematically modulating
gene expression in both loss-of-function and gain-of-function experiments. We describe …