… (CL Vizcarra and SL Mayo, unpublished results). Pokala and Handel [48 • ] have adapted
the GB method of Brooks and co-workers [49] for use in protein design. They overcome the …

Three semi-rational approaches, combinatorial site-saturation mutagenesis (CSSM) using a
reduced amino acid set and two libraries based on C orbit and CRAM computational design …

Evidence for cooperation between actin nucleators is growing. The WH2-containing nucleator
Spire and the formin Cappuccino interact directly, and both are essential for assembly of …

To determine which of seven library design algorithms best introduces new protein function
without destroying it altogether, seven combinatorial libraries of green fluorescent protein …

Metallothioneins (MTs) are a ubiquitous class of small metal-binding proteins involved in
metal homeostasis and detoxification. While known for their high affinity for d10 metal ions, …

Formins are multidomain proteins that assemble actin in a wide variety of biological processes.
They both nucleate and remain processively associated with growing filaments, in some …

Formin family actin nucleators are potential coordinators of the actin and microtubule
cytoskeletons, as they can both nucleate actin filaments and bind microtubules in vitro. To gain a …

Successfully modeling electrostatic interactions is one of the key factors required for the
computational design of proteins with desired physical, chemical, and biological properties. In …

Formins are a conserved family of proteins known to enhance actin polymerization. Most
formins are regulated by an intramolecular interaction. The Drosophila formin, Cappuccino (…

The N‐end rule pathway uses an evolutionarily conserved mechanism in bacteria and
eukaryotes that marks proteins for degradation by ATP‐dependent chaperones and proteases …