… Similarly, Cas4 that is present in subtypes IA-D and II-B has been proposed to be involved
in the CRISPR adaptation process, and this prediction has been validated experimentally for …

… (B) Schematic illustrating the plasmid depletion assay for discovering the PAM position
and identity. Competent E. coli harboring either the heterologous FnCpf1 locus plasmid (pFnCpf1…

… (B) Heat maps showing indel percentage of guide sequences with a single-base mismatch.
(C) Indel formation with guide sequences containing consecutive transversion mismatches. …

… b, Indels at Pcsk9 targets in liver tissue following injection of AAV at 2 × 10 11 total genome
copies (n = 3 animals). c, d, Time course of serum Pcsk9 (c) and total cholesterol in animals (…

… (b) In vitro processing of FnCpf1 pre-crRNA transcript (80 nM) with purified AsCpf1 or … (c)
RNA-seq analysis of FnCpf1 pre-crRNA cleavage products, as shown in b. A high fraction of …

… (b) ASCL1 expression measured by qPCR in HEK293FT cells transfected with split-4 (Split)
and four sgRNAs per gene. Expression was measured in cells with and without rapamycin (n …

… two b strands that form a small antiparallel sheet (Figures S3A and S3B). Domains A and B
… Thus, domains A, B, and C are referred to as the WED, PI, and Nuc domains, respectively. …

… -Cre-generated deletion allele (B, BamHI site). (B) Southern blot analysis of homologous …
BamHI-digested DNA was probed with probe B; 15-kb, 11-kb, and 7.5-kb bands represent …

… a twisted fivestranded b sheet flanked by four a helices, and is responsible for the recognition
of the distorted repeat:anti-repeat duplex, as described above (Figures 4B and 4E and …

… identified 27 members of the Cas12b family that are encoded within type V–B loci. The type
V–B systems are widely scattered among bacteria, and topology of the phylogenetic tree of …