This overview evaluates the capabilities of mass spectrometry (MS) in combination with gas
chromatography (GC) and liquid chromatography (LC) for the determination of a multitude of …

Single-cell RNA sequencing (scRNA-seq) offers new possibilities to address biological and
medical questions. However, systematic comparisons of the performance of diverse scRNA-…

… The diet containing 0.1% mineral oil of type A or B led to an absorption rate in rats of 52%
and 35%, respectively, whereas in pigs, the percentages were 38% and 20%, respectively. …

… Christoph Ziegenhain, Beate Vieth and Swati Parekh authors contributed equally to this …
Beate Vieth studied biology in Leuven and applied statistics in Ghent until 2012 and is doing her …

… B) to confirm that the cutoff used by zUMIs is valid for a given dataset. In cases where the
number of BCs or BC sequences are known, it is preferable to use this information. If zUMIs is ei…

… b The discriminatory ability determined by the partial area under the curve (mean pAUC
± sd) based on DE testing with limma-trend for normalisation without spike-ins per DE-pattern. …

… In panel (b), we report the marginal power to detect a log2-fold change of 0.5 and in panel
(d) the corresponding FDR, whereas the nominal FDR was set to α = 0.05 (dashed line). In …

… b cDNA yield dependent on the absence (gray) or presence (blue) of 7.5% PEG 8000 during
reverse transcription and template switching. Shown are three independent reactions for …

… (B) These distribution parameters are then used to set-up the simulations. For better
comparability, the parameters for the simulation of differential expression are set separately. (C) …

… B To determine if intronic reads stem from genomic DNA or mRNA, we extracted DNA from
mouse embryonic stem cells (mESCs) and RNA from human-induced pluripotent stem cells (…