User profiles for B. Spanjaard

Bastiaan Spanjaard

Postdoctoral researcher, BIMSB, MDC Berlin
Verified email at mdc-berlin.de
Cited by 2218

Single-cell sequencing reveals dissociation-induced gene expression in tissue subpopulations

SC van den Brink, F Sage, Á Vértesy, B Spanjaard… - Nature …, 2017 - nature.com
b. SCs were sorted from a single cell suspension of 6 TA muscles isolated from 3 mice
via FACS. Left panel: live cells were selected based on Hoechst staining and Forward …

Simultaneous lineage tracing and cell-type identification using CRISPR–Cas9-induced genetic scars

B Spanjaard, B Hu, N Mitic, P Olivares-Chauvet… - Nature …, 2018 - nature.com
… More precisely, the chance of forming a connection between cell type A and B is given by
p(A–B) = 2 * CE(A)* CE(B)/CE(tot) 2 , and that of forming a connection within cell type A by p(A–…

Integrated genome and transcriptome sequencing of the same cell

SS Dey, L Kester, B Spanjaard, M Bienko… - Nature …, 2015 - nature.com
… (b) Distribution of reads within 100 nucleotides of Dppa5a for two cells as a function of the
random priming location by Ad-2. The unique length-based identifiers found in the two cells …

[HTML][HTML] Origin and function of activated fibroblast states during zebrafish heart regeneration

B Hu, S Lelek, B Spanjaard, H El-Sammak… - Nature …, 2022 - nature.com
The adult zebrafish heart has a high capacity for regeneration following injury. However, the
composition of the regenerative niche has remained largely elusive. Here, we dissected the …

GPSeq reveals the radial organization of chromatin in the cell nucleus

…, T Kallas, F Agostini, E Wernersson, B Spanjaard… - Nature …, 2020 - nature.com
… Next, we examined how A and B compartments defined by Hi… very central, whereas A and
B compartments had a similar radial … at the level of A and B subcompartments 38 , given that …

Methods for lineage tracing on the organism-wide level

B Spanjaard, JP Junker - Current opinion in cell biology, 2017 - Elsevier
… (b) Genetic labeling of cells with the Cre/lox system provides a high level of control and is …
in the desired cells and their progeny (Figure 2b). The fact that cell labeling can be performed …

[HTML][HTML] Spatio-temporal mRNA tracking in the early zebrafish embryo

…, P Drewe-Boß, J Mintcheva, B Spanjaard… - Nature …, 2021 - nature.com
b Histogram shows raw transcript counts per section. c Tomo-seq tracks for the known
vegetally localized genes dazl, trim36, celf1, wnt8a, and grip2a and whole-mount in situ …

Postnatal cardiac gene editing using CRISPR/Cas9 with AAV9-mediated delivery of short guide RNAs results in mosaic gene disruption

…, AR Leitoguinho, C Demkes, B Spanjaard… - Circulation …, 2017 - Am Heart Assoc
Rationale: CRISPR/Cas9 (clustered regularly interspaced palindromic repeats/CRISPR-associated
protein 9)–based DNA editing has rapidly evolved as an attractive tool to modify the …

[PDF][PDF] Spatial transcriptomics of C. elegans males and hermaphrodites identifies sex-specific differences in gene expression patterns

A Ebbing, Á Vértesy, MC Betist, B Spanjaard… - Developmental Cell, 2018 - cell.com
To advance our understanding of the genetic programs that drive cell and tissue specialization,
it is necessary to obtain a comprehensive overview of gene expression patterns. Here, …

Massively parallel whole-organism lineage tracing using CRISPR/Cas9 induced genetic scars

…, B Spanjaard, J Peterson-Maduro, A Alemany, B Hu… - Biorxiv, 2016 - biorxiv.org
… (b). Correlation of scar abundances between two different 24 hpf embryos injected with …
(b). Representative examples of scar frequencies for two individual embryos derived from a …