User profiles for B. Spanjaard
Bastiaan SpanjaardPostdoctoral researcher, BIMSB, MDC Berlin Verified email at mdc-berlin.de Cited by 2218 |
Single-cell sequencing reveals dissociation-induced gene expression in tissue subpopulations
… b. SCs were sorted from a single cell suspension of 6 TA muscles isolated from 3 mice
via FACS. Left panel: live cells were selected based on Hoechst staining and Forward …
via FACS. Left panel: live cells were selected based on Hoechst staining and Forward …
Simultaneous lineage tracing and cell-type identification using CRISPR–Cas9-induced genetic scars
B Spanjaard, B Hu, N Mitic, P Olivares-Chauvet… - Nature …, 2018 - nature.com
… More precisely, the chance of forming a connection between cell type A and B is given by
p(A–B) = 2 * CE(A)* CE(B)/CE(tot) 2 , and that of forming a connection within cell type A by p(A–…
p(A–B) = 2 * CE(A)* CE(B)/CE(tot) 2 , and that of forming a connection within cell type A by p(A–…
Integrated genome and transcriptome sequencing of the same cell
… (b) Distribution of reads within 100 nucleotides of Dppa5a for two cells as a function of the
random priming location by Ad-2. The unique length-based identifiers found in the two cells …
random priming location by Ad-2. The unique length-based identifiers found in the two cells …
[HTML][HTML] Origin and function of activated fibroblast states during zebrafish heart regeneration
B Hu, S Lelek, B Spanjaard, H El-Sammak… - Nature …, 2022 - nature.com
The adult zebrafish heart has a high capacity for regeneration following injury. However, the
composition of the regenerative niche has remained largely elusive. Here, we dissected the …
composition of the regenerative niche has remained largely elusive. Here, we dissected the …
GPSeq reveals the radial organization of chromatin in the cell nucleus
… Next, we examined how A and B compartments defined by Hi… very central, whereas A and
B compartments had a similar radial … at the level of A and B subcompartments 38 , given that …
B compartments had a similar radial … at the level of A and B subcompartments 38 , given that …
Methods for lineage tracing on the organism-wide level
B Spanjaard, JP Junker - Current opinion in cell biology, 2017 - Elsevier
… (b) Genetic labeling of cells with the Cre/lox system provides a high level of control and is …
in the desired cells and their progeny (Figure 2b). The fact that cell labeling can be performed …
in the desired cells and their progeny (Figure 2b). The fact that cell labeling can be performed …
[HTML][HTML] Spatio-temporal mRNA tracking in the early zebrafish embryo
…, P Drewe-Boß, J Mintcheva, B Spanjaard… - Nature …, 2021 - nature.com
… b Histogram shows raw transcript counts per section. c Tomo-seq tracks for the known
vegetally localized genes dazl, trim36, celf1, wnt8a, and grip2a and whole-mount in situ …
vegetally localized genes dazl, trim36, celf1, wnt8a, and grip2a and whole-mount in situ …
Postnatal cardiac gene editing using CRISPR/Cas9 with AAV9-mediated delivery of short guide RNAs results in mosaic gene disruption
…, AR Leitoguinho, C Demkes, B Spanjaard… - Circulation …, 2017 - Am Heart Assoc
Rationale: CRISPR/Cas9 (clustered regularly interspaced palindromic repeats/CRISPR-associated
protein 9)–based DNA editing has rapidly evolved as an attractive tool to modify the …
protein 9)–based DNA editing has rapidly evolved as an attractive tool to modify the …
[PDF][PDF] Spatial transcriptomics of C. elegans males and hermaphrodites identifies sex-specific differences in gene expression patterns
A Ebbing, Á Vértesy, MC Betist, B Spanjaard… - Developmental Cell, 2018 - cell.com
To advance our understanding of the genetic programs that drive cell and tissue specialization,
it is necessary to obtain a comprehensive overview of gene expression patterns. Here, …
it is necessary to obtain a comprehensive overview of gene expression patterns. Here, …
Massively parallel whole-organism lineage tracing using CRISPR/Cas9 induced genetic scars
…, B Spanjaard, J Peterson-Maduro, A Alemany, B Hu… - Biorxiv, 2016 - biorxiv.org
… (b). Correlation of scar abundances between two different 24 hpf embryos injected with …
(b). Representative examples of scar frequencies for two individual embryos derived from a …
(b). Representative examples of scar frequencies for two individual embryos derived from a …