[HTML][HTML] High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs
TP Huang, ZJ Heins, SM Miller, BG Wong… - Nature …, 2023 - nature.com
Despite the availability of Cas9 variants with varied protospacer-adjacent motif (PAM)
compatibilities, some genomic loci—especially those with pyrimidine-rich PAM sequences—…
compatibilities, some genomic loci—especially those with pyrimidine-rich PAM sequences—…
Precise, automated control of conditions for high-throughput growth of yeast and bacteria with eVOLVER
Precise control over microbial cell growth conditions could enable detection of minute
phenotypic changes, which would improve our understanding of how genotypes are shaped by …
phenotypic changes, which would improve our understanding of how genotypes are shaped by …
[HTML][HTML] Prospective isolation of NKX2-1–expressing human lung progenitors derived from pluripotent stem cells
…, AN Hollenberg, S Nguyen, BG Wong… - The Journal of …, 2017 - Am Soc Clin Investig
It has been postulated that during human fetal development, all cells of the lung epithelium
derive from embryonic, endodermal, NK2 homeobox 1–expressing (NKX2-1 + ) precursor …
derive from embryonic, endodermal, NK2 homeobox 1–expressing (NKX2-1 + ) precursor …
Functional genomics of the rapidly replicating bacterium Vibrio natriegens by CRISPRi
The fast-growing Gram-negative bacterium Vibrio natriegens is an attractive microbial system
for molecular biology and biotechnology due to its remarkably short generation time 1 , 2 …
for molecular biology and biotechnology due to its remarkably short generation time 1 , 2 …
A biomineral-inspired approach of synthesizing colloidal persistent phosphors as a multicolor, intravital light source
Many in vivo biological techniques, such as fluorescence imaging, photodynamic therapy,
and optogenetics, require light delivery into biological tissues. The limited tissue penetration …
and optogenetics, require light delivery into biological tissues. The limited tissue penetration …
Microfabrication of high-resolution porous membranes for cell culture
Microporous membranes are widely utilized in cell biology to study cell–cell signaling and
cell migration. However, the thickness and low porosity of commercial track-etched …
cell migration. However, the thickness and low porosity of commercial track-etched …
[HTML][HTML] Coordinated regulation of acid resistance in Escherichia coli
…, AI Stettner, J Wang, BG Wong, WS Wong… - BMC systems …, 2017 - Springer
Background Enteric Escherichia coli survives the highly acidic environment of the stomach
through multiple acid resistance (AR) mechanisms. The most effective system, AR2, …
through multiple acid resistance (AR) mechanisms. The most effective system, AR2, …
Automated Continuous Evolution of Proteins in Vivo
Z Zhong, BG Wong, A Ravikumar… - ACS synthetic …, 2020 - ACS Publications
We present automated continuous evolution (ACE), a platform for the hands-free directed
evolution of biomolecules. ACE pairs OrthoRep, a genetic system for continuous targeted …
evolution of biomolecules. ACE pairs OrthoRep, a genetic system for continuous targeted …
Vibrio natriegens, a new genomic powerhouse
Recombinant DNA technology has revolutionized biomedical research with continual
innovations advancing the speed and throughput of molecular biology. Nearly all these tools, …
innovations advancing the speed and throughput of molecular biology. Nearly all these tools, …
Designing automated, high-throughput, continuous cell growth experiments using eVOLVER
ZJ Heins, CP Mancuso, S Kiriakov, BG Wong… - JoVE (Journal of …, 2019 - jove.com
Continuous culture methods enable cells to be grown under quantitatively controlled
environmental conditions, and are thus broadly useful for measuring fitness phenotypes and …
environmental conditions, and are thus broadly useful for measuring fitness phenotypes and …