We use in situ Hi-C to probe the 3D architecture of genomes, constructing haploid and diploid
maps of nine cell types. The densest, in human lymphoblastoid cells, contains 4.9 billion …

We recently used in situ Hi-C to create kilobase-resolution 3D maps of mammalian genomes.
Here, we combine these maps with new Hi-C, microscopy, and genome-editing …

The human genome folds to create thousands of intervals, called "contact domains," that
exhibit enhanced contact frequency within themselves. "Loop domains" form because of …

Activation of the μ-opioid receptor (μOR) is responsible for the efficacy of the most effective
analgesics. To shed light on the structural basis for μOR activation, here we report a 2.1 Å X-…

Cohesin extrusion is thought to play a central role in establishing the architecture of mammalian
genomes. However, extrusion has not been visualized in vivo, and thus, its functional …

During interphase, the inactive X chromosome (Xi) is largely transcriptionally silent and
adopts an unusual 3D configuration known as the “Barr body.” Despite the importance of X …

Understanding how a subset of expressed genes dictates cellular phenotype is a considerable
challenge owing to the large numbers of molecules involved, their combinatorics and the …

… To understand why this is the case, Sanborn et al. analyzed the genome of baker’s yeast …
Sanborn et al. then developed a machine learning algorithm to predict activation domains in …

Chromatin loops are a major component of 3D nuclear organization, visually apparent as
intense point-to-point interactions in Hi-C maps. Identification of these loops is a critical part of …

RNA-based medicines and RNA-targeting drugs are emerging as promising new approaches
for treating disease. Optimizing these therapeutics by naive experimental screening is a …