Mapping the genomic landscape of CRISPR–Cas9 cleavage

…, E Lau, LM Banh, AM Lied, LS Edwards, AH Settle… - Nature …, 2017 - nature.com
RNA-guided CRISPR–Cas9 endonucleases are widely used for genome engineering, but
our understanding of Cas9 specificity remains incomplete. Here, we developed a biochemical …

SHAPE-Seq 2.0: systematic optimization and extension of high-throughput chemical probing of RNA secondary structure with next generation sequencing

D Loughrey, KE Watters, AH Settle… - Nucleic acids …, 2014 - academic.oup.com
RNA structure is a primary determinant of its function, and methods that merge chemical
probing with next generation sequencing have created breakthroughs in the throughput and …

A perception-based nanosensor platform to detect cancer biomarkers

Z Yaari, Y Yang, E Apfelbaum, C Cupo, AH Settle… - Science …, 2021 - science.org
Conventional molecular recognition elements, such as antibodies, present issues for
developing biomolecular assays for use in certain technologies, such as implantable devices. …

[HTML][HTML] NmeCas9 is an intrinsically high-fidelity genome-editing platform

…, A Gupta, KE Sasaki, T Wu, PD Donohoue, AH Settle… - Genome Biology, 2018 - Springer
Background The development of CRISPR genome editing has transformed biomedical
research. Most applications reported thus far rely upon the Cas9 protein from Streptococcus …

[HTML][HTML] Characterizing RNA structures in vitro and in vivo with selective 2′-hydroxyl acylation analyzed by primer extension sequencing (SHAPE-Seq)

KE Watters, MY Angela, EJ Strobel, AH Settle, JB Lucks - Methods, 2016 - Elsevier
RNA molecules adopt a wide variety of structures that perform many cellular functions,
including, among others, catalysis, small molecule sensing, and cellular defense. Our ability to …

[HTML][HTML] SITE-Seq: A genome-wide method to measure Cas9 cleavage

AP May, P Cameron, AH Settle, CK Fuller… - 2017 - researchsquare.com
CRISPR-Cas9 RNA-guided endonucleases are widely used in genome engineering, yet
information on biochemical and cellular off-target cleavage activity is lacking. Here, we present …

[HTML][HTML] Plasma membrane abundance dictates phagocytic capacity and functional crosstalk in myeloid cells

BY Winer, AH Settle, AM Yakimov, C Jeronimo… - bioRxiv, 2023 - ncbi.nlm.nih.gov
Professional phagocytes like neutrophils and macrophages tightly control what they eat,
how much they eat, and when they move after eating. We show that plasma membrane …

[HTML][HTML] Topographical analysis of immune cell interactions reveals a biomechanical signature for immune cytolysis

M de Jesus, AH Settle, D Vorselen, TK Gaetjens… - bioRxiv, 2023 - ncbi.nlm.nih.gov
Immune cells live intensely physical lifestyles characterized by structural plasticity,
mechanosensitivity, and force exertion. Whether specific immune functions require stereotyped …

Inheritance settlement of descendants of children and siblings in Islamic law with local wisdom in Indonesia

S Khosyi'ah, AY Rusyana - Cogent Social Sciences, 2022 - Taylor & Francis
… Sunnah of the Prophet because it is the settlement of property-related inheritance cases. If
… Unlike the case with inheritance law which is based on local culture, the settlement is based …

β2 integrins impose a mechanical checkpoint on macrophage phagocytosis

AH Settle, BY Winer, MM de Jesus, L Seeman, Z Wang… - bioRxiv, 2024 - biorxiv.org
Phagocytosis is an intensely physical process that depends on the mechanical properties of
both the phagocytic cell and its chosen target. Here, we employed differentially deformable …