… Genetic experiments discussed below indicate that this binding site is functional in vivo (Schier
and Gehring, 1993a). To avoid artificial dimerization of the homeodomain polypeptide …

▪ Abstract TGFß signals belonging to the Nodal family set up the embryonic axes, induce
mesoderm and endoderm, pattern the nervous system, and determine left-right asymmetry in …

Non-coding RNAs (ncRNAs) are emerging as key regulators of embryogenesis. They
control embryonic gene expression by several means, ranging from microRNA-induced …

Spatial localization is a key determinant of cellular fate and behavior, but methods for spatially
resolved, transcriptome-wide gene expression profiling across complex tissues are lacking…

Systematic genome-wide mutagenesis screens for embryonic phenotypes have been
instrumental in the understanding of invertebrate and plant development. Here, we report the …

MicroRNAs (miRNAs) comprise 1 to 3% of all vertebrate genes, but their in vivo functions
and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the …

MicroRNAs (miRNAs) are small RNAs that regulate gene expression posttranscriptionally.
To block all miRNA formation in zebrafish, we generated maternal-zygotic dicer (MZdicer) …

The CRISPR/Cas9 system has been implemented in a variety of model organisms to mediate
site-directed mutagenesis. A wide range of mutation rates has been reported, but at a …

Long noncoding RNAs (lncRNAs) comprise a diverse class of transcripts that structurally
resemble mRNAs but do not encode proteins. Recent genome-wide studies in humans and the …

The zebrafish EGF-CFC gene one-eyed pinhead (oep) is required zygotically for the
formation of the ventral neuroectoderm, endoderm, and prechordal plate. Here we report that …