Chitooligosaccharides (CHOS) are homo- or heterooligomers of N-acetylglucosamine and D-glucosamine.
CHOS can be produced using chitin or chitosan as a starting material, using …

During the past 15 years there has been a continuous flow of reports describing proteins
stabilized by the introduction of mutations. These reports span a period from pioneering rational …

Enzymes currently known as lytic polysaccharide monooxygenases (LPMOs) play an
important role in the conversion of recalcitrant polysaccharides, but their mode of action has …

Modern enzyme development relies to an increasing extent on strategies based on diversity
generation followed by screening for variants with optimised properties. In principle, these …

Bacterial proteins categorized as family 33 carbohydrate‐binding modules (CBM33) were
recently shown to cleave crystalline chitin, using a mechanism that involves hydrolysis and …

Efficient enzymatic conversion of crystalline polysaccharides is crucial for an economically
and environmentally sustainable bioeconomy but remains unfavorably inefficient. We …

Lactic acid bacteria (LAB) fight competing Gram-positive microorganisms by secreting anti-microbial
peptides called bacteriocins. Peptide bacteriocins are usually divided into …

Chitinase B (ChiB) from Serratia marcescens is a family 18 exo-chitinase whose catalytic
domain has a TIM-barrel fold with a tunnel-shaped active site. We have solved structures of …

The Gram-negative soil bacterium Serratia marcescens uses three different family 18
chitinases to degrade chitin, an abundant insoluble carbohydrate polymer composed of β(1,4)-…

The recently discovered lytic polysaccharide monooxygenases (LPMOs) are known to carry
out oxidative cleavage of glycoside bonds in chitin and cellulose, thus boosting the activity of …