[HTML][HTML] A consensus genome-scale reconstruction of Chinese hamster ovary cell metabolism
Chinese hamster ovary (CHO) cells dominate biotherapeutic protein production and are widely
used in mammalian cell line engineering research. To elucidate metabolic bottlenecks in …
used in mammalian cell line engineering research. To elucidate metabolic bottlenecks in …
[HTML][HTML] PinAPL-Py: A comprehensive web-application for the analysis of CRISPR/Cas9 screens
Large-scale genetic screens using CRISPR/Cas9 technology have emerged as a major tool
for functional genomics. With its increased popularity, experimental biologists frequently …
for functional genomics. With its increased popularity, experimental biologists frequently …
A Markov chain model for N-linked protein glycosylation–towards a low-parameter tool for model-driven glycoengineering
PN Spahn, AH Hansen, HG Hansen, J Arnsdorf… - Metabolic …, 2016 - Elsevier
Glycosylation is a critical quality attribute of most recombinant biotherapeutics. Consequently,
drug development requires careful control of glycoforms to meet bioactivity and biosafety …
drug development requires careful control of glycoforms to meet bioactivity and biosafety …
ZNF263 is a transcriptional regulator of heparin and heparan sulfate biosynthesis
RJ Weiss, PN Spahn, AG Toledo… - Proceedings of the …, 2020 - National Acad Sciences
Heparin is the most widely prescribed biopharmaceutical in production globally. Its potent
anticoagulant activity and safety makes it the drug of choice for preventing deep vein …
anticoagulant activity and safety makes it the drug of choice for preventing deep vein …
Restoration of DNA repair mitigates genome instability and increases productivity of Chinese hamster ovary cells
… Spahn and Xiaolin Zhang performed and analyzed all remaining experiments. … Philipp
N. Spahn and Xiaolin Zhang contributed equally to this study. …
N. Spahn and Xiaolin Zhang contributed equally to this study. …
Genome-wide screens uncover KDM2B as a modifier of protein binding to heparan sulfate
Heparan sulfate (HS) proteoglycans bind extracellular proteins that participate in cell signaling,
attachment and endocytosis. These interactions depend on the arrangement of sulfated …
attachment and endocytosis. These interactions depend on the arrangement of sulfated …
Systems glycobiology for glycoengineering
PN Spahn, NE Lewis - Current opinion in biotechnology, 2014 - Elsevier
… Author links open overlay panel Philipp N Spahn 1 , Nathan E Lewis 2 … Protein glycosylation
involves the covalent addition of glycans to the amino group of an asparagine (N-linked) or …
involves the covalent addition of glycans to the amino group of an asparagine (N-linked) or …
Predictive glycoengineering of biosimilars using a Markov chain glycosylation model
Biosimilar drugs must closely resemble the pharmacological attributes of innovator products
to ensure safety and efficacy to obtain regulatory approval. Glycosylation is one critical …
to ensure safety and efficacy to obtain regulatory approval. Glycosylation is one critical …
A metabolic CRISPR-Cas9 screen in Chinese hamster ovary cells identifies glutamine-sensitive genes
Media and feed optimization have fueled many-fold improvements in mammalian
biopharmaceutical production, but genome editing offers an emerging avenue for further enhancing …
biopharmaceutical production, but genome editing offers an emerging avenue for further enhancing …
Modulating carbohydrate–protein interactions through glycoengineering of monoclonal antibodies to impact cancer physiology
… Author links open overlay panel Austin WT Chiang 1 2 , Shangzhong Li 1 2 3 , Philipp N
Spahn 1 2 , Anne Richelle 1 2 , Chih-Chung Kuo 1 2 3 , Mojtaba Samoudi 1 2 , Nathan E Lewis …
Spahn 1 2 , Anne Richelle 1 2 , Chih-Chung Kuo 1 2 3 , Mojtaba Samoudi 1 2 , Nathan E Lewis …