Cytoplasmic dynein is a homodimeric AAA+ motor that transports a multitude of cargos
toward the microtubule minus end. How the two catalytic head domains interact and move …

A filamentous cytoskeleton largely governs the physical shape and mechanical properties
of eukaryotic cells. In bacteria, proteins homologous to all three classes of eukaryotic …

Complex spatial and temporal patterns of gene expression underlie embryo differentiation,
yet methods do not yet exist for the efficient genome-wide determination of spatial expression …

Reproductive isolation is a key component of speciation. In many insects, a major driver of
this isolation is cuticular hydrocarbon pheromones, which help to identify potential …

Patterning in the Drosophila melanogaster embryo is affected by multiple maternal factors, but
the effect of these factors on spatial gene expression has not been systematically analyzed…

Whole-genome sequencing, particularly in fungi, has progressed at a tremendous rate. More
difficult, however, is experimental testing of the inferences about gene function that can be …

Spatial patterning of gene expression is a key process in development, yet how it evolves is
still poorly understood. Both cis- and trans-acting changes could participate in complex …

Recently, a number of protocols extending RNA-sequencing to the single-cell regime have
been published. However, we were concerned that the additional steps to deal with such …

We developed a protocol to measure the bending rigidity of filamentous rod-shaped bacteria.
Forces are applied with an optical trap, a microscopic three-dimensional spring made of …

Pheromones known as cuticular hydrocarbons are a major component of reproductive isolation
in Drosophila. Individuals from morphologically similar sister species produce different …