Oct4 and Nanog are transcription factors required to maintain the pluripotency and self-renewal
of embryonic stem (ES) cells. Using the chromatin immunoprecipitation paired-end …

DNA methylation and chromatin modification are two global mechanisms that regulate gene
expression. Recent studies provide insight into the mechanism of transcriptional silencing …

Detection of new genomic control elements is critical in understanding transcriptional regulatory
networks in their entirety. We studied the genome-wide binding locations of three key …

Transcription factors (TFs) and their specific interactions with targets are crucial for specifying
gene-expression programs. To gain insights into the transcriptional regulatory networks in …

Mammalian DNA is methylated at many CpG dinucleotides. The biological consequences of
methylation are mediated by a family of methyl-CpG binding proteins 1, 2, 3, 4. The best …

Reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) can be achieved
by viral-mediated transduction of defined transcription factors. Moving toward the eventual …

Over the past few years, the long-standing idea that covalent modification of chromatin can
play a role in determining states of gene activity has been confirmed. Eukaryotic genes can …

Cytosine residues in the sequence 5′CpG (cytosine–guanine) are often postsynthetically
methylated in animal genomes. CpG methylation is involved in long-term silencing of certain …

The ability to derive a whole-genome map of transcription-factor binding sites (TFBS) is
crucial for elucidating gene regulatory networks. Herein, we describe a robust approach that …

ATP-dependent nucleosome remodeling and core histone acetylation and deacetylation
represent mechanisms to alter nucleosome structure. NuRD is a multisubunit complex …