ALTHOUGH mitotic cyclins are well-known substrates for ubiquitin-mediated proteolysis at
the metaphase–anaphase transition 1–4 , their degradation is not essential for separation of …

Tri-methylation of histone H3 lysine 9 is important for recruiting heterochromatin protein 1 (HP1)
to discrete regions of the genome, thereby regulating gene expression, chromatin …

Successful cell division requires the precise and timely coordination of chromosomal,
cytoskeletal and membrane trafficking events. These processes are regulated by the competing …

The movement of chromosomes that precedes meiosis was observed in living cells of fission
yeast by fluorescence microscopy. Further analysis by in situ hybridization revealed that the …

The spindle assembly checkpoint (SAC) delays anaphase onset until kinetochores accomplish
bioriented microtubule attachments [1]. Although several centromeric and kinetochore …

Fluorescence in situ hybridization (FISH) shows that fission yeast centromeres and telomeres
make up specific spatial arrangements in the nucleus. Their positioning and clustering are …

A hallmark of mitosis is the appearance of high levels of histone phosphorylation, yet the
roles of these modifications remain largely unknown. Here, we demonstrate that histone H3 …

In cells lacking centrosomes, such as those found in female meiosis, chromosomes must
nucleate and stabilize microtubules in order to form a bipolar spindle. Here we report the …

Structural heterogeneity of nucleosomes in functional chromosomes is unknown. Here, we
devise the template-, reference- and selection-free (TRSF) cryo-EM pipeline to …

At anaphase, the linkage between sister chromatids is dissolved and the separated sisters
move toward opposite poles of the spindle. We developed a method to purify metaphase and …