Although the functional interaction between ubiquitin-conjugating enzymes (E2s) and ubiquitin
ligases (E3s) is essential in ubiquitin (Ub) signalling, the criteria that define an active E2–…

Ubiquitination is a post-translational modification pathway involved in myriad cellular
regulation and disease pathways. The Ub (ubiquitin) transfer cascade requires three enzyme …

Ubiquitin signaling pathways rely on E3 ligases for effecting the final transfer of ubiquitin from
E2 ubiquitin conjugating enzymes to a protein target. Here we re-evaluate the hybrid RING/…

10.7554/eLife.06547.001 The endosomal sorting complexes required for transport (ESCRT)
machinery mediates the physical separation between daughter cells during cytokinetic …

Ubiquitination of the αN-terminus of protein substrates has been reported sporadically since
the early 1980s. However, the identity of an enzyme responsible for this unique ubiquitin (Ub…

Cytokinetic abscission facilitates the irreversible separation of daughter cells. This process
requires the endosomal-sorting complexes required for transport (ESCRT) machinery and is …

The 12 related human ESCRT-III proteins form filaments that constrict membranes and
mediate fission, including during cytokinetic abscission. The C-terminal tails of polymerized …

Proper neuronal development is essential to growth and adult brain function. Alterations at
any step of this highly organized sequence of events, due to genetic mutations or …

The endosomal sorting complex required for transport (ESCRT) pathway remodels
membranes during multivesicular body biogenesis, the abscission stage of cytokinesis, and …

The biochemical and structural characterization of ubiquitin-conjugating enzymes (E2s)
over the past 30 years has fostered important insights into ubiquitin transfer mechanisms. …