RNA promotes liquid-liquid phase separation (LLPS) to build membraneless compartments
in cells. How distinct molecular compositions are established and maintained in these liquid …

Conspectus RNA molecules convey biological information both in their linear sequence and
in their base-paired secondary and tertiary structures. Chemical probing experiments, which …

We report that the SARS-CoV-2 nucleocapsid protein (N-protein) undergoes liquid-liquid
phase separation (LLPS) with viral RNA. N-protein condenses with specific RNA genomic …

Eukaryotes possess a vast array of RNA-binding proteins (RBPs) that affect mRNAs in
diverse ways to control protein expression. Combinatorial regulation of mRNAs by RBPs is …

PUF proteins are a conserved family of eukaryotic RNA-binding proteins that regulate
specific mRNAs: they control many processes including stem cell proliferation, fertility, and …

7SK is a conserved noncoding RNA that regulates transcription by sequestering the
transcription factor P-TEFb. 7SK function entails complex changes in RNA structure, but …

Chemical probing is an important tool for characterizing the complex folded structures of
RNA molecules, many of which play key cellular roles. Electrophilic SHAPE reagents create …

PUF proteins are potent repressors that serve important roles in stem cell maintenance,
neurological processes, and embryonic development. These functions are driven by PUF protein …

RNA–protein interaction networks govern many biological processes but are difficult to
examine comprehensively. We devised ribonucleoprotein networks analyzed by mutational …

10.7554/eLife.17096.001 Collaboration among the multitude of RNA-binding proteins (RBPs)
is ubiquitous, yet our understanding of these key regulatory complexes has been limited to …