Correlated movement and bending of nucleic acid structures visualized by multicolor single-molecule spectroscopy

Chemphyschem. 2009 Jul 13;10(9-10):1455-60. doi: 10.1002/cphc.200900109.

Abstract

At the end of the rainbow.... The structure and dynamics of three- and four- way DNA junctions are studied using triple alternating laser excitation. Steroid binding distorts the structure of three-way junctions with correlated bending of all branches towards a steeper pyramid (see picture). In four-way junctions, the dynamics of three distances are visualized simultaneously.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry*
  • DNA, Cruciform / chemistry*
  • Fluorescence Resonance Energy Transfer
  • Lasers
  • Nucleic Acid Conformation
  • Spectrometry, Fluorescence
  • Steroids / chemistry

Substances

  • DNA, Cruciform
  • Steroids
  • DNA