Quantitative evaluation of the lengths of homobifunctional protein cross-linking reagents used as molecular rulers

Protein Sci. 2001 Jul;10(7):1293-304. doi: 10.1110/ps.51201.

Abstract

Homobifunctional chemical cross-linking reagents are important tools for functional and structural characterization of proteins. Accurate measures of the lengths of these molecules currently are not available, despite their widespread use. Stochastic dynamics calculations now provide quantitative measures of the lengths, and length dispersions, of 32 widely used molecular rulers. Significant differences from published data have been found.

Supplemental material: See www.proteinscience.org

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amines / chemistry
  • Cross-Linking Reagents / chemistry*
  • Models, Molecular*
  • Molecular Conformation
  • Molecular Structure
  • Proteins / chemistry*
  • Stochastic Processes
  • Sulfhydryl Compounds / chemistry

Substances

  • Amines
  • Cross-Linking Reagents
  • Proteins
  • Sulfhydryl Compounds