Abstract
Rationale
Relapse to drug-seeking in abstinent heroin addicts and reinstatement in experimental animals are observed when exposed to drug-associated stimuli or cues, the drug itself, and stressful events. It has been shown that footshock-induced stress increases the rewarding effects of opiates, delays extinction, and induces the reinstatement of drug-seeking. However, the effects of social stress on the reinstatement of opiate-seeking after extinction has not been studied.
Objectives
The role of physical (restraint and tail pinch) and social (social defeat) stressors on the reinstatement of morphine-induced conditioned place preference (CPP) was evaluated.
Methods
Adult male OF1 mice were conditioned with 10, 20, or 40 mg/kg of morphine or saline. Only morphine-conditioned animals acquired CPP. All mice underwent extinction sessions until the CPP was extinguished. Then, the effects of physical or social stress on the reinstatement of CPP were evaluated. Morphine- and saline-conditioned animals were exposed to the respective stressor or control stress condition immediately or 15 min before reinstatement tests. In experiment 1, animals underwent restraint for 15 min. In experiment 2, animals were exposed to tail pinch or placed in a cage without any manipulation for 15 min. In experiment 3, animals performed an agonistic encounter with an isolated or anosmic mouse or were placed in a cage without any social contact or manipulation.
Results
Restraint, tail pinch, and social defeat in an agonistic encounter with an isolated mouse produce the reinstatement of CPP in morphine-conditioned animals.
Conclusions
These data demonstrate that social stress is as effective as physical stress in reinstating morphine-seeking.
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Acknowledgements
This work was supported by the following grants: Conselleria de Sanitat, Centro Superior de Investigación en Salud Publica, Generalitat Valenciana and Ministerio de Sanidad y Consumo, Instituto de Salud “Carlos III” (FIS), Redes Temáticas de Investigación Cooperativa (G03/005), and Ministerio de Educación y Ciencia and Fondo Europeo de Desarrollo Regional (ref. BSO2002-00106) (Spain). Bruno Ribeiro Do Couto was supported by grant SFRH/BD/4559/2001 of the Fundação Para a Ciência e a Tecnologia, Ministério da Ciência e da Tecnologia (Portugal). We wish to thank Javier Carrasco (Servei d´Endocrinologia i Radioimmunoanàlisi, Universitat Autònoma de Barcelona) for the analysis of corticosterone, Israel Alberola for the technical support for the investigation, and Ms. Miriam Phillips for the English revision of the manuscript.
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Ribeiro Do Couto, B., Aguilar, M.A., Manzanedo, C. et al. Social stress is as effective as physical stress in reinstating morphine-induced place preference in mice. Psychopharmacology 185, 459–470 (2006). https://doi.org/10.1007/s00213-006-0345-z
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DOI: https://doi.org/10.1007/s00213-006-0345-z