LOCUS pEJ03_mCitrine_KanMX_HO 7085 bp ds-DNA circular 29-DEC-2016 DEFINITION . FEATURES Location/Qualifiers promoter 5144..6131 /label="prACT1" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd gene complement(1552..2412) /label="AmpR" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 2666..3495 /label="HOl" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 3908..4717 /label="KanMX" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e promoter 3529..3907 /label="pTEF" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 175..667 /label="HOr" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 promoter complement(68..88) /label="pT7" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 promoter 5008..5030 /label="pSP6" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 primer_bind complement(6459..6482) /label="oMM0032 3'" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Origin of Replication 778..1397 /label="pUC Ori" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer_bind 5107..5126 /label="oJHK267 5' end" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 terminator 4726..4950 /label="tTEF" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 CDS 6132..6848 /label="ymCitrineM233I" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd terminator 6849..7085 /label="ADH1 terminator" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd ORIGIN 1 aacgagctcg aattcatcga tgatatcaga tccactagtg gcctatgcgg ccgcggatct 61 gccggtctcc ctatagtgag tcgtattaat ttcgataagc caggttaacc tgcattaatg 121 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctgggg 181 gaacaacttc acagaatgtt ttgtcatatt gtcgaagtgg tcacaaaaca agagaagttc 241 cgccaattat aaaaagggaa cccgtatatt tcagcttcac ggatgatttc cagggtgaga 301 gtactgtata tgggcttacg atagaaggcc ataaaaattt cttgcttggc aacaaaatag 361 aagtgaaatc atgtcgaggc tgctgtgtgg gagaacagct taaaatatca caaaaaaaga 421 atctaaaaca ctgtgttgct tgtcccagaa agggaatcaa gtatttttat aaagattgga 481 gtggtaaaaa tcgagtatgt gctagatgct atggaagata caaattcagc ggtcatcact 541 gtataaattg caagtatgta ccagaagcac gtgaagtgaa aaaggcaaaa gacaaaggcg 601 aaaaattggg cattacgccc gaaggtttgc cagttaaagg accagagtgt ataaaatgtg 661 gcggaattgt acagtttaaa caaaggcggt aatacggtta tccacagaat caggggataa 721 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 781 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 841 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 901 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 961 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 1021 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 1081 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 1141 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 1201 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 1261 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 1321 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 1381 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 1441 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 1501 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 1561 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 1621 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 1681 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 1741 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 1801 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 1861 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 1921 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 1981 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 2041 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 2101 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 2161 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 2221 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 2281 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 2341 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 2401 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 2461 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 2521 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 2581 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 2641 cctctgacac atgcagctgt ttaaacgcaa gcacaaaaaa tcaaggagag agaactacca 2701 ctttgttcat gtgtacaatg ttcattatct ccataagcaa aaaaaaaaaa tagaaaacat 2761 atgctataag gttgatattc tcacgagtaa gcggcacttg ctacttattg acattgcaga 2821 tttttggcta cagaaatagt atattagaga ttataattgc taatcaaatc aaaatataaa 2881 attagtaaac caaaccattt atacccttcc ttagtagtta tggattgttt tttaatgata 2941 tttctgcaaa ccaaagaaag attgttatcc agatagaatt tagttttgat attcattttt 3001 ttgttgaaga ttgaacgcca tatctgggcc tcataattca aaagacggtg ccattatcgg 3061 tagcgtttcg cattgtactg gatttcagaa atttcacagt tgatgaatcg aaaagaatgg 3121 tctcattgca acacgtaagg ttaagatgtc cctttttacc attataggca ataaatgaat 3181 cataaaacga ccgtatactg gtgaaatagt agggagaacg agtacctgta gtaaaaagta 3241 taaatcatag ttaatcgggc aatgtccctc gatcaaggag tattgtgtca tgttcgagac 3301 aaacgccaac atttttgttt cttttggaca aatgttgttt gcatttatga tccgttatat 3361 tttgatctaa tgtagagttg cacgtagttc ttactggcaa agaaatcgat gcataccaaa 3421 aaagaataaa ggtgatattt gatctttacc gtttagttcc aacgtaaaat tgtgcctttg 3481 gacttaaaat ggcgttcaga gcagattgta ctgagagtgc acctgtttag cttgcctcgt 3541 ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg 3601 acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc 3661 atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg 3721 gctcctcgct gcagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc 3781 cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct 3841 ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata 3901 aacaaccatg ggtaaggaaa agactcacgt ttcgaggccg cgattaaatt ccaacatgga 3961 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat 4021 ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag 4081 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc 4141 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc 4201 gatccccggc aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat 4261 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc 4321 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt 4381 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa 4441 agaaatgcat aagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc 4501 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt 4561 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc 4621 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa 4681 attgcagttt catttgatgc tcgatgagtt tttctaatca gtactgacaa taaaaagatt 4741 cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt tctattttaa 4801 tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc agatgcgaag 4861 ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt cgctatactg 4921 ctgtcgattc gatactaacg ccgccatcca gtgtcgaaaa cgcgcggcta caattaatac 4981 ataaccttat Tgtatcatac acatacgatt taggtgacac tatagaacgc ggccgccagc 5041 tgaagcttac accatggaat ctagaatctc gagaaggtac cagagctagc atactgcagg 5101 tcgacgCGTA CGCTGCAGGT CGACGGATCC CCGGGttaat taaGTGGCTG CAAGAGATTG 5161 ATCGGTATGC AACCTCAACA GTGTTGAAGC TATTGGTAGG TAACAAGTGT GATTTAAAGG 5221 ACAAGCGTGT CGTGGAATAT GACGTGGCAA AGGAATTTGC GGACGCGAAT AAGATGCCGT 5281 TCTTAGAGAC TAGTGCTTTG GACTCCACCA ACGTCGAGGA TGCGTTTTTA ACCATGGCTA 5341 GACAAATCAA GGAAAGTATG TCCCAACAAA ACCTGAACGA AACCACTCAG AAGAAGGAAG 5401 ACAAAGGGAA CGTCAACCTG AAGGGACAGA GTTTAACCAA CACCGGTGGG GGCTGCTGTT 5461 GAACAAGCGC GCCTCTACCT TGCAGACCCA TATAATATAA TAACTAAATA AGTAAATAAG 5521 ACACACGCGA GAACATATAT ACACAATTAC AGTAACAATA ACAAGAGGAC AGATACTACC 5581 AAAATGTGTG GGGAAGCGGG TAAGCTGCCA CAGCAATTAA TGCACAACAT TTAACCTACA 5641 TTCTTCCTTA TCGGATCCTC AAAACCCTTA AAAACATATG CCTCACCCTA ACATATTTTC 5701 CAATTAACCC TCAATATTTC TCTGTCACCC GGCCTCTATT TTCCATTTTC TTCTTTACCC 5761 GCCACGCGTT TTTTTCTTTC AAATTTTTTT CTTCCTTCTT CTTTTTCTTC CACGTCCTCT 5821 TGCATAAATA AATAAACCGT TTTGAAACCA AACTCGCCTC TCTCTCTCCT TTTTGAAATA 5881 TTTTTGGGTT TGTTTGATCC TTTCCTTCCC AATCTCTCTT GTTTAATATA TATTCATTTA 5941 TATCACGCTC TCTTTTTATC TTCCTTTTTT TCCTCTCTCT TGTATTCTTC CTTCCCCTTT 6001 CTACTCAAAC CAAGAAGAAA AAGAAAAGGT CAATCTTTGT TAAAGAATAG GATCTTCTAC 6061 TACATCAGCT TTTAGATTTT TCACGCTTAC TGCTTTTTTC TTCCCAAGAT CGAAAATTTA 6121 CTGAATTAAC Aatgtctaaa ggtgaagaat tattcactgg tgttgtccca attttggttg 6181 aattagatgg tgatgttaat ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg 6241 ctacttacgg taaattgacc ttaaaattta tttgtactac tggtaaattg ccagttccat 6301 ggccaacctt agtcactact ttaggttatg gtttgatgtg ttttgctaga tacccagatc 6361 atatgaaaca acatgacttt ttcaagtctg ccatgccaga aggttatgtt caagaaagaa 6421 ctattttttt caaagatgac ggtaactaca agaccagagc tgaagtcaag tttgaaggtg 6481 ataccttagt taatagaatc gaattaaaag gtattgattt taaagaagat ggtaacattt 6541 taggtcacaa attggaatac aactataact ctcacaatgt ttacatcatg gctgacaaac 6601 aaaagaatgg tatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggttctgttc 6661 aattagctga ccattatcaa caaaatactc caattggtga tggtccagtc ttgttaccag 6721 acaaccatta cttatcctat caatctagat tatccaaaga tccaaacgaa aagagagacc 6781 acatggtctt gttagaattt gttactgctg ctggtattac ccatggtatt gatgaattgt 6841 acaaataagg cgcgccACTT CTAAATAAGC GAATTTCTTA TGATTTATGA TTTTTATTAT 6901 TAAATAAGTT ATAAAAAAAA TAAGTGTATA CAAATTTTAA AGTGACTCTT AGGTTTTAAA 6961 ACGAAAATTC TTATTCTTGA GTAACTCTTT CCTGTAGGTC AGGTTGCTTT CTCAGGTATA 7021 GTATGAGGTC GCTCTTATTG ACCACACCTC TACCGGCAGA TCCGCTAGGG ATAACAGGGT 7081 AATAT //