TY - JOUR T1 - When Wavelengths Collide: Bias in Cell Abundance Measurements due to Expressed Fluorescent Proteins JF - bioRxiv DO - 10.1101/037010 SP - 037010 AU - Ariel Hecht AU - Drew Endy AU - Marc Salit AU - Matthew S. Munson Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/01/16/037010.abstract N2 - The abundance of bacteria in liquid culture is commonly inferred by measuring optical density at 600 nm. Red fluorescent proteins (RFP) can strongly absorb light at 600 nm. Increasing RFP expression can falsely inflate apparent cell density and lead to underestimations of mean per-cell fluorescence by up to 10%. Measuring optical density at 700 nm would allow estimation of cell abundance unaffected by the presence of nearly all fluorescent proteins. ER -