RT Journal Article
SR Electronic
T1 Nested metabarcode tagging: a robust tool for studying species interactions in ecology and evolution
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 035071
DO 10.1101/035071
A1 James JN Kitson
A1 Christoph Hahn
A1 Richard J Sands
A1 Nigel A Straw
A1 Darren M Evans
A1 David H Lunt
YR 2015
UL http://biorxiv.org/content/early/2015/12/23/035071.abstract
AB Here we present a next generation sequencing approach for use in ecological studies which allows for individual level sample tracking through the use of hierarchically organised molecular identification tags. We demonstrate its utility on a sample data set examining levels of parasitism in a recently arrived invasive species in the UK and discuss further possibilities for our approach in ecology and evolution.