PT - JOURNAL ARTICLE AU - Mohammed OE Abdallah AU - Ubai K Algizouli AU - Maram Abbas Suliman AU - Rawya Abdulaziz Abdulrahman AU - Mahmoud Koko AU - Ghimja Fessahaye AU - Jamal Haleem Shakir AU - Ahmed H. Fahal AU - Ahmed M Elhassan AU - Muntaser E Ibrahim AU - Hiba S Mohamed TI - Methylome analysis reveals dysregulated developmental and viral pathways in breast cancer AID - 10.1101/034322 DP - 2015 Jan 01 TA - bioRxiv PG - 034322 4099 - http://biorxiv.org/content/early/2015/12/13/034322.short 4100 - http://biorxiv.org/content/early/2015/12/13/034322.full AB - Background Breast cancer (BC) ranks among the most common cancers in Sudan and worldwide with hefty toll on female health and human resources. Recent studies have uncovered a common BC signature characterized by low frequency of oncogenic mutations and high frequency of epigenetic silencing of major BC tumor suppressor genes. Therefore, we conducted a genome-wide methylome study to characterize aberrant DNA methylation in breast cancer.Results Differential methylation analysis between primary tumor samples and normal samples from healthy adjacent tissues yielded 20188 differentially methylated positions (DMPs), which is further divided into 13633 hypermethylated sites corresponding to 5339 genes and 6555 hypomethylated sites corresponding to 2811 genes. Moreover, bioinformatics analysis revealed epigenetic dysregulation of major developmental pathways including hippo signaling pathway. We also uncovered many clues to a possible role for EBV infection in BCConclusion Our results clearly show the utility of epigenetic assays in interrogating breast cancer tumorigenesis, and pinpointing specific developmental and viral pathways dysregulation that might serve as potential biomarkers or targets for therapeutic interventions.