TY - JOUR T1 - CrispRVariants: precisely charting the mutation spectrum in genome engineering experiments JF - bioRxiv DO - 10.1101/034140 SP - 034140 AU - Helen Lindsay AU - Alexa Burger AU - Anastasia Felker AU - Christopher Hess AU - Jonas Zaugg AU - Elena Chiavacci AU - Carolin Anders AU - Martin Jinek AU - Christian Mosimann AU - Mark D. Robinson Y1 - 2015/01/01 UR - http://biorxiv.org/content/early/2015/12/10/034140.abstract N2 - CRISPR-Cas9 and related technologies efficiently alter genomic DNA at targeted positions and have far-reaching implications for functional screening and therapeutic gene editing. Understanding and unlocking this potential requires accurate evaluation of editing efficiency. We show that methodological decisions for analyzing sequencing data can significantly affect mutagenesis efficiency estimates and we provide a comprehensive R-based toolkit, CrispRVariants, that resolves and localizes individual mutant alleles with respect to the endonuclease cut site. CrispRVariants-enabled analyses of newly generated and existing genome editing datasets underscore how careful consideration of the full variant spectrum gives insight toward effective guide and amplicon design as well as the mutagenic process. ER -