PT  - JOURNAL ARTICLE
AU  - Carolina Vera
AU  - Miguel Barría
TI  - Effect of Poli I:C in murine model of experimental autoimmune thyroiditis
AID  - 10.1101/034066
DP  - 2015 Jan 01
TA  - bioRxiv
PG  - 034066
4099  - http://biorxiv.org/content/early/2015/12/09/034066.short
4100  - http://biorxiv.org/content/early/2015/12/09/034066.full
AB  - Toll-like receptors (TLR) detect pathogen-associated molecular patters, expressed by bacterias, virus and fungi. TLR are expressed principally in antigen presenting cells (APC). Upon activation the TLR signalling results in the production of several proinflammatory cytokines subsequently inducing an adaptative immune response. The aim of this study was to evaluate mRNA of the TLR3, TLR9 and cytokines (IFN-beta, TNF and IL-1) in thyroid follicular cells obtained of mice with autoimmune experimental thyroiditis (EAT). For that C57BL/6 mice were immunized with Tg plus complete Freund’s adyuvant and treated or not with Poli I:C. Anti-thyroglobulin autoantibodies level was determined by indirect ELISA. Also isolated thyroid follicles were treated in vitro with Poli IC. The results show the animals with TAE present humoral and cellular autoimmune response. TLR3 receptors and cytokines (IFN-beta, TNF and IL-10) are more highly expressed in thyroid follicular cells treated with Poli I:C. Taken together, these results suggest that TLR3 expression in thyroid follicular cells induces signaling mechanisms to enhanced IL-1, TNF and IFN-β levels during pathogen infections and that could trigger of autoimmune thyroiditis.