@article {Baker032714, author = {Perrin Baker and Preston J. Hill and Brendan D. Snarr and Noor Alnabelseya and Mathew J. Pestrak and Mark J. Lee and Laura K. Jennings and John Tam and Roman Melnyk and Matthew R. Parsek and Donald C. Sheppard and Daniel J. Wozniak and P. Lynne Howell}, title = {Exopolysaccharide biosynthetic glycoside hydrolases can be utilized to disrupt and prevent Pseudomonas aeruginosa biofilms}, elocation-id = {032714}, year = {2015}, doi = {10.1101/032714}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Bacterial biofilms are a significant medical challenge as they are recalcitrant to current therapeutic regimes. A key component of biofilm formation in the opportunistic human pathogen Pseudomonas aeruginosa is the biosynthesis of the exopolysaccharides Pel and Psl, which are involved in the formation and maintenance of the structural biofilm scaffold and protection against antimicrobials and host defenses. Given that the glycoside hydrolases {\textendash} PelAh and PslGh {\textendash} encoded in the pel and psl biosynthetic operons, respectively, are utilized for in vivo exopolysaccharide processing, we reasoned that these would provide specificity to target P. aeruginosa biofilms. Evaluating these enzymes as potential therapeutics, we demonstrate that these glycoside hydrolases selectively target and degrade the exopolysaccharide component of the biofilm matrix and that nanomolar concentrations of these enzymes can both prevent biofilm formation as well as rapidly disrupt preexisting biofilms in vitro. This treatment was effective against clinical and environmental P. aeruginosa isolates and reduced biofilm biomass by 58{\textendash}94\%. These non-cytotoxic enzymes potentiated antibiotics as the addition of either enzyme to a sub-lethal concentration of colistin reduced viable bacterial counts by 2.5 orders of magnitude. Additionally, PelAh was able to increase neutrophil killing by ~50\%. This work illustrates the feasibility and benefits of using bacterial exopolysaccharide biosynthetic glycoside hydrolases and synthetic biology to develop novel anti-biofilm therapeutics.}, URL = {https://www.biorxiv.org/content/early/2015/11/26/032714}, eprint = {https://www.biorxiv.org/content/early/2015/11/26/032714.full.pdf}, journal = {bioRxiv} }