RT Journal Article SR Electronic T1 G-quadruplex prediction in E.coli genome reveals a conserved putative G-quadruplex-Hairpin-Duplex switch JF bioRxiv FD Cold Spring Harbor Laboratory SP 032615 DO 10.1101/032615 A1 Osman Doluca A1 Oktay I Kaplan A1 Burak Berber A1 Nezih Hekim YR 2015 UL http://biorxiv.org/content/early/2015/11/24/032615.abstract AB Many studies show that short non-coding sequences are widely conserved among regulatory elements. More and more conserved sequences are being discovered since the development next generation sequencing technology. A common approach to identify conserved sequences with regulatory roles rely on the topological change such as hairpin formation on the DNA or RNA level. However, G-quadruplexes, a non-cannonical nucleic acid topology with little established biological role, is rarely considered for conserved regulatory element discovery. Here we present the use of G-quadruplex prediction algorithm to identify putative G-quadruplex-forming and conserved elements in E.coli genome. Phylogenetic analysis of 52 G-quadruplex forming sequences revealed two conserved G-quadruplex motifs with potential regulatory role.