TY - JOUR T1 - G-quadruplex prediction in <em>E.coli</em> genome reveals a conserved putative G-quadruplex-Hairpin-Duplex switch JF - bioRxiv DO - 10.1101/032615 SP - 032615 AU - Osman Doluca AU - Oktay I Kaplan AU - Burak Berber AU - Nezih Hekim Y1 - 2015/01/01 UR - http://biorxiv.org/content/early/2015/11/24/032615.abstract N2 - Many studies show that short non-coding sequences are widely conserved among regulatory elements. More and more conserved sequences are being discovered since the development next generation sequencing technology. A common approach to identify conserved sequences with regulatory roles rely on the topological change such as hairpin formation on the DNA or RNA level. However, G-quadruplexes, a non-cannonical nucleic acid topology with little established biological role, is rarely considered for conserved regulatory element discovery. Here we present the use of G-quadruplex prediction algorithm to identify putative G-quadruplex-forming and conserved elements in E.coli genome. Phylogenetic analysis of 52 G-quadruplex forming sequences revealed two conserved G-quadruplex motifs with potential regulatory role. ER -