TY - JOUR T1 - A Genetic Screen Identifies Two Novel Rice Cysteine-rich Receptor-like Kinases That Are Required for the Rice NH1-mediated Immune Response JF - bioRxiv DO - 10.1101/003129 SP - 003129 AU - Mawsheng Chern AU - Rebecca S. Bart AU - Wei Bai AU - Deling Ruan AU - Wing Hoi Sze-To AU - Patrick E. Canlas AU - Rashmi Jain AU - Xuewei Chen AU - Pamela Ronald Y1 - 2014/01/01 UR - http://biorxiv.org/content/early/2014/02/28/003129.abstract N2 - AbbreviationsBTHbenzothiadiazoleCRKcysteine-rich receptor-like kinasesNH1NPR1 homolog 1NPR1non-expressor of pathogenesis-related genes 1SAsalicylic acidSARsystemic acquired resistance; sn11, suppressor of NH1-mediated lesion mimics 1.Over-expression of rice NH1 (NH1ox), the ortholog of Arabidopsis NPR1, confers immunity to bacterial and fungal pathogens and induces the appearance of necrotic lesions due to activation of defense genes at the pre-flowering stage. This lesion-mimic phenotype can be enhanced by the application of benzothiadiazole (BTH). To identify genes regulating these responses, we screened a fast neutron-irradiated NH1ox rice population. We identified one mutant, called sn11 (suppressor of NH1-mediated lesion-mimic 1), which is impaired both in BTH-induced necrotic lesion formation and in the immune response. Using a comparative genome hybridization approach employing rice whole genome tiling array, we identified 11 genes associated with the sn11 phenotype. Transgenic analysis revealed that RNA interference of two of the genes, encoding previously uncharacterized cysteine-rich receptor-like kinases (CRK6 and CRK10), re-created the sn11 phenotype. Elevated expression of CRK10 using an inducible expression system resulted in enhanced immunity. Quantitative PCR revealed that BTH treatment and elevated levels of rice NH1 and its paralog NH3 induced expression of CRK10 and CRK6 RNA. These results indicate that CRK6 and CRK10 are required for the BTH-activated immune response mediated by NH1. ER -