RT Journal Article SR Electronic T1 Blood circulation in the tunicate Corella inflata (Corellidae) JF bioRxiv FD Cold Spring Harbor Laboratory SP 029322 DO 10.1101/029322 A1 Michael W. Konrad YR 2015 UL http://biorxiv.org/content/early/2015/10/16/029322.abstract AB The ascidian tunicate Corella inflata is relatively transparent compared to other solitary tunicates and the circulatory system can be visualized by injecting high molecular weight fluorescein labeled dextran into the beating heart or the large vessels at the ends of the heart. In addition, after staining with neutral red the movement of blood cells can be followed to further define and characterize the circulatory system. The heart is a gently curved tube with a constriction in the middle and extends across the width of the animal. As in other tunicates, pumping is peristaltic and periodically reverses direction. During the abvisceral directional phase blood leaves the anterior end of the heart in two asymmetric vessels that connect to the two sides of the branchial basket (or pharynx), in contrast to the direct connection between the heart and the endostyle seen in the commonly studied tunicate Ciona intestinalis. In Corella inflata blood then flows in both transverse directions through a complex system of ducts in the branchial basket into large ventral and dorsal vessels and then to the visceral organs in the posterior of the animal. During the advisceral phase blood leaves the posterior end of the heart in vessels that repeatedly bifurcate to fan into the stomach and gonads. Blood speed, determined by following individual cells, is high and pulsatory near the heart, but decreases and becomes more constant in peripheral regions. Estimated blood flow volume during one directional phase is greater than the total volume of the animal. Circulating blood cells are confined to vessels or ducts in the visible parts of the animal and retention of high molecular weight dextran in the vessels is comparable to that seen in vertebrates. These flow patterns are consistent with a closed circulatory network.