TY - JOUR T1 - Single molecule sequencing of THCA synthase reveals copy number variation in modern drug-type <em>Cannabis sativa</em> L JF - bioRxiv DO - 10.1101/028654 SP - 028654 AU - Kevin J. McKernan AU - Yvonne Helbert AU - Vasisht Tadigotla AU - Stephen McLaughlin AU - Jessica Spangler AU - Lei Zhang AU - Douglas Smith Y1 - 2015/01/01 UR - http://biorxiv.org/content/early/2015/10/08/028654.abstract N2 - Cannabinoid expression is an important genetically determined feature of cannabis that presents clinical and legal implications for patients seeking cannabinoid specific therapies like Cannabidiol (CBD).Cannabinoid, terpenoid, and flavonoid marker assisted selection can accelerate breeding efforts by offering genetic tools to select for desired traits at an early stage in growth. To this end, multiple models for chemotype inheritance have been described suggesting a complex picture for chemical phenotype determination.Here we explore the potential role of copy number variation of THCA Synthase using phased single molecule sequencing and demonstrate that copy number and sequence variation of this gene is common and suggests a more nuanced view of chemotype prediction. ER -