RT Journal Article
SR Electronic
T1 A genome-wide resource for the analysis of protein localisation in <em>Drosophila</em>
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 028308
DO 10.1101/028308
A1 Mihail Sarov
A1 Chritiane Barz
A1 Helena Jambor
A1 Marco Y. Hein
A1 Christopher Schmied
A1 Dana Suchold
A1 Bettina Stender
A1 Stephan Janosch
A1 Vinay K.J. Vikas
A1 R.T. Krisnan
A1 K. Aishwarya
A1 Irene R.S. Ferreira
A1 Radoslaw K. Ejsmont
A1 Katja Finkl
A1 Susanne Hasse
A1 Philipp Kämpfer
A1 Nicole Plewka
A1 Elisabeth Vinis
A1 Siegfried Schloissnig
A1 Elisabeth Knust
A1 Volker Hartenstein
A1 Matthias Mann
A1 Mani Ramaswami
A1 K. VijayRaghavan
A1 Pavel Tomancak
A1 Frank Schnorrer
YR 2015
UL http://biorxiv.org/content/early/2015/10/04/028308.abstract
AB The Drosophila genome contains &gt;13,000 protein coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here we present a genome-wide fosmid library of ≈10,000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins we have created transgenic lines and for a total of 207 lines we have assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we can visualise many proteins at endogenous expression levels and find a large fraction of them localising to subcellular compartments. Using complementation tests we demonstrate that two-thirds of the tagged proteins are fully functional. Moreover, our clones also enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will enable systematic analysis of protein expression and localisation in various cellular and developmental contexts.