TY - JOUR T1 - A genome-wide resource for the analysis of protein localisation in <em>Drosophila</em> JF - bioRxiv DO - 10.1101/028308 SP - 028308 AU - Mihail Sarov AU - Chritiane Barz AU - Helena Jambor AU - Marco Y. Hein AU - Christopher Schmied AU - Dana Suchold AU - Bettina Stender AU - Stephan Janosch AU - Vinay K.J. Vikas AU - R.T. Krisnan AU - K. Aishwarya AU - Irene R.S. Ferreira AU - Radoslaw K. Ejsmont AU - Katja Finkl AU - Susanne Hasse AU - Philipp Kämpfer AU - Nicole Plewka AU - Elisabeth Vinis AU - Siegfried Schloissnig AU - Elisabeth Knust AU - Volker Hartenstein AU - Matthias Mann AU - Mani Ramaswami AU - K. VijayRaghavan AU - Pavel Tomancak AU - Frank Schnorrer Y1 - 2015/01/01 UR - http://biorxiv.org/content/early/2015/10/04/028308.abstract N2 - The Drosophila genome contains &gt;13,000 protein coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here we present a genome-wide fosmid library of ≈10,000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins we have created transgenic lines and for a total of 207 lines we have assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we can visualise many proteins at endogenous expression levels and find a large fraction of them localising to subcellular compartments. Using complementation tests we demonstrate that two-thirds of the tagged proteins are fully functional. Moreover, our clones also enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will enable systematic analysis of protein expression and localisation in various cellular and developmental contexts. ER -