%0 Journal Article %A Durafshan Sakeena Syed %A BM Swetha %A O. Venkateswara Reddy %A Heinrich Reichert %A VijayRaghavan K. %T Glial and neuronal Semaphorin signaling instruct the development of a functional myotopic map for Drosophila walking %D 2015 %R 10.1101/027375 %J bioRxiv %P 027375 %X Motoneurons developmentally acquire appropriate cellular architectures that ensure connections with postsynaptic muscles and presynaptic neurons. In Drosophila, leg motoneurons are organized as a myotopic map, where their dendritic domains represent the muscle field. Here we investigate mechanisms underlying development of aspects of this myotopic map, required for walking. A behavioral screen identified roles for Semaphorins (Serna) and Plexins (Plex) in walking behavior. Deciphering this phenotype, we show that PlexA/Sema1a mediates motoneuron axon branching in ways that differ in the proximal femur and distal tibia, based on motoneuronal birth order. Importantly, we show a novel role for glia in positioning dendrites of specific motoneurons; PlexB/Sema2a is required for dendritic positioning of late-born motoneurons but not early-born motoneurons. These findings indicate that communication within motoneurons and between glia and motoneurons, mediated by the combined action of different Plexin/Semaphorin signaling systems, are required for the formation of a functional myotopic map. %U https://www.biorxiv.org/content/biorxiv/early/2015/09/22/027375.full.pdf