RT Journal Article
SR Electronic
T1 Complete assembly of novel environmental bacterial genomes by MinION<sup>TM</sup> sequencing
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 026930
DO 10.1101/026930
A1 Daniel J Turner
A1 Xiaoguang Dai
A1 Simon C Mayes
A1 Sissel Juul
YR 2015
UL http://biorxiv.org/content/early/2015/09/19/026930.abstract
AB In this study, we adapt a protocol for the growth of previously uncultured environmental bacterial isolates, to make it compatible with whole genome sequencing. We demonstrate that in combination with the MinION sequencing device, complete assemblies can be derived, allowing genomic comparisons to be made. This approach allows rapid, inexpensive and straightforward discovery, and genomic analysis, of previously uncultured prokaryotic genomes, and brings greater ownership of all parts of the sequencing process back to individual researchers.