PT  - JOURNAL ARTICLE
AU  - Ghazaleh Afshar
AU  - Ahmed El Hady
AU  - Theo Geisel
AU  - Walter Stuehmer
AU  - Fred Wolf
TI  - Enhancing burst activation and propagation in cultured neuronal networks by photo-stimulation
AID  - 10.1101/027177
DP  - 2015 Jan 01
TA  - bioRxiv
PG  - 027177
4099  - http://biorxiv.org/content/early/2015/09/18/027177.short
4100  - http://biorxiv.org/content/early/2015/09/18/027177.full
AB  - Spontaneous bursting activity in cultured neuronal networks is initiated by leader neurons, which constitute a small subset of first-to-fire neurons forming a sub-network that recruits follower neurons into the burst. While the existence and stability of leader neurons is well established, the influence of stimulation on the leader-follower dynamics is not sufficiently understood. By combining multi-electrode array recordings with whole field optical stimulation of cultured Channelrhodopsin-2 transduced hippocampal neurons, we show that fade-in photo-stimulation induces a significant shortening of intra-burst firing rate peak delay of follower electrodes after offset of the stimulation compared to unperturbed spontaneous activity. Our study shows that optogenetic stimulation can be used to change the dynamical fine structure of self-organized network bursts.