PT - JOURNAL ARTICLE AU - Di Wu AU - Haitao Li AU - Yufeng Liu AU - Hexige Saiyin AU - Chenji Wang AU - Zhen Wei AU - Wenjiao Zen AU - Danyang Liu AU - Qi Chen AU - Haojie Huang AU - Guoyuan Liu AU - Songmin Jiang AU - Long Yu TI - ZNF191 Inhibits Hepatocellular Carcinoma Metastasis via DLG1-mediated YAP1 Inactivation AID - 10.1101/027060 DP - 2015 Jan 01 TA - bioRxiv PG - 027060 4099 - http://biorxiv.org/content/early/2015/09/18/027060.short 4100 - http://biorxiv.org/content/early/2015/09/18/027060.full AB - Searching targets for hepatocellular carcinoma (HCC) treatment, we identified zinc finger protein 191 (ZNF191) as a suppressor against HCC metastasis. Over-expressing ZNF191 in HCC cells impaired cell motility, while ZNF191 depletion promoted HCC cell migration in vitro and metastasis in vivo through triggering yes-associated protein 1 (YAP1) signaling. Chromatin immunoprecipitation-sequencing (ChIP-seq) revealed that ZNF191 specifically bound to the promoter of Discs, Large homolog 1 (DLG1), a cell polarity maintainer and a negative regulator of YAP1. Double-knockdown experiments showed that DLG1 was not only the mediator of ZNF191’s function to suppress migration but also a link between ZNF191 and YAP1 signaling. ZNF191 was down-regulated in metastatic HCCs, correlating positively with DLG1 levels and inversely with YAP1 activation. Our findings indicate ZNF191 functions as a metastasis suppressor via DLG1-mediated YAP1 signaling inactivation.