TY - JOUR T1 - Single-cell phenotyping of human induced pluripotent stem cells by high-throughput imaging JF - bioRxiv DO - 10.1101/026955 SP - 026955 AU - H. Christian Volz AU - Florian Heigwer AU - Tatjana Wüest AU - Marta Galach AU - Jochen Utikal AU - Hugo A. Katus AU - Michael Boutros Y1 - 2015/01/01 UR - http://biorxiv.org/content/early/2015/09/16/026955.abstract N2 - Single-cell phenotyping promises to yield insights into biological responses in heterogeneous cell populations. We developed a method based on single-cell analysis to phenotype human induced pluripotent stem cells (hIPSC) by high-throughput imaging. Our method uses markers for morphology and pluripotency as well as social features to characterize perturbations using a meta-phenotype based on mapping single cells to distinct phenotypic classes. Analysis of perturbations on a single cell level enhances the applicability of human induced pluripotent stem cells (hIPSC) for screening experiments taking the inherently increased phenotypic variability of these cells into account. We adapted miniaturized culture conditions to allow for the utilization of hIPSC in RNA interference (RNAi) high-throughput screens and single cell phenotyping by image analysis. We identified key regulators of pluripotency in hIPSC masked in a population-averaged analysis and we confirmed several candidate genes (SMG1, TAF1) and assessed their effect on pluripotency. ER -