TY - JOUR T1 - Immune stimulation reduces sleep and memory ability in <em>Drosophila melanogaster</em> JF - bioRxiv DO - 10.1101/002717 SP - 002717 AU - E. B. Mallon AU - A. Alghamdi AU - R. T. K. Holdbrook AU - E. Rosato Y1 - 2014/01/01 UR - http://biorxiv.org/content/early/2014/02/13/002717.abstract N2 - Psychoneuroimmunology studies the increasing number of connections between neurobiology and immunology. We establish Drosophila melanogaster as a tractable model in this field by demonstrating the effects of the immune response on two fundamental behaviours: sleep and memory ability.We used the Geneswitch system to upregulate peptidoglycan receptor protein (PGRP) expression, thereby stimulating the immune system in the absence of infection. Geneswitch was activated by feeding the steroid RU486, to the flies. Importantly, by stimulating the immune system of adult flies in the absence of infection we have avoided the added complications of developmental and disease effects that have confounded other studies. We used an aversive classical conditioning paradigm to quantify memory and measures of activity to infer sleep.Immune stimulated flies exhibited reduced levels of sleep, which could not be explained by a generalised increase in waking activity. The effects on sleep were more pronounced for day compared to night sleep. Immune stimulated flies also showed a reduction in memory abilities.These are important results as they establish Drosophila as a model for immune-neural interactions and provide a possible role for sleep in the interplay between the immune response and memory. ER -