TY - JOUR T1 - Genome-wide characterization of PRE-1 reveals a hidden evolutionary relationship between suidae and primates JF - bioRxiv DO - 10.1101/025791 SP - 025791 AU - Hao Yu AU - Qingyan Wu AU - Jing Zhang AU - Ying Zhang AU - Chao Lu AU - Yunyun Cheng AU - Zhihui Zhao AU - Andreas Windemuth AU - Di Liu AU - Linlin Hao Y1 - 2015/01/01 UR - http://biorxiv.org/content/early/2015/08/31/025791.abstract N2 - We identified and characterized a free PRE-1 element inserted into the promoter region of the porcine IGFBP7 gene whose integration mechanisms into the genome, including copy number, distribution preferences, capacity to exonize and phyloclustering pattern are similar to that of the primate Alu element. 98% of these PRE-1 elements also contain two conserved internal AluI restriction enzyme recognition sites, and the RNA structure of PRE1 can be folded into a two arms model like the Alu RNA structure. It is more surprising that the length of the PRE-1 fragments is nearly the same in 20 chromosomes and positively correlated to its fracture site frequency. All of these fracture sites are close to the mutation hot spots of PRE-1 families, and most of these hot spots are located in the non-complementary fragile regions of the PRE-1 RNA structure. Sequence homology analysis showed that the PRE-1 element seemed to share a common ancestor 7SL RNA with primates but was generated by different evolutionary model, which suggests that the suidae may be the closest relatives to primates in laurasiatheria. ER -