TY - JOUR T1 - Transcriptomic Characterization of Soybean Roots in Response to <em>Bradyrhizobium</em> Infection by RNA Sequencing JF - bioRxiv DO - 10.1101/024224 SP - 024224 AU - Qingyuan He AU - Yang Hongyan AU - Ping Wu AU - Zhengpeng Li AU - Songhua Wang AU - Changwei Zhu Y1 - 2015/01/01 UR - http://biorxiv.org/content/early/2015/08/20/024224.abstract N2 - Legumes interact with rhizobium convert N2 into ammonia for plant use. To investigate the plant basal nitrogen fixation mechanisms induced in response to Bradyrhizobium, differential gene expression in root of inoculated and mock-inoculated soybean was analysed by RNA-Seq. A total of 55787 transcripts were aligned to soybean genome reference sequences, 280 and 316 transcripts were found to be up-and down-regulated, respectively, in inoculated relative to mock-inoculated soybean’s root at V1 stage. Gene ontology (GO) analyses detected 104, 182 and 178 genes associated with cell component category, molecular function category and biological process category, respectively. Pathway analysis revealed that 98 differentiallly expressed genes (115 transcripts) involved in 169 biological pathways. We selected 19 differentially expressed genes and analyzed their expressions in mock-inoculated, inoculated USDA110 and CCBAU45436 using qRT-PCR. The results were consistent with those obtained from rhizobia infected RNA-Seq data. These showed that the results of RNA-Seq have reliability and universality. Additionally, this study showed some novel genes associated with nitrogen fixation process comparison with the previously identified QTLs. ER -