RT Journal Article SR Electronic T1 Tools and pipelines for BioNano data: molecule assembly pipeline and FASTA super scaffolding tool JF bioRxiv FD Cold Spring Harbor Laboratory SP 020966 DO 10.1101/020966 A1 Jennifer M Shelton A1 Michelle C Coleman A1 Nic Herndon A1 Nanyan Lu A1 Ernest T Lam A1 Thomas Anantharaman A1 Palak Sheth A1 Susan J Brown YR 2015 UL http://biorxiv.org/content/early/2015/08/18/020966.abstract AB Background Genome assembly remains an unsolved problem. Assembly projects face a range of hurdles that confound assembly. Thus a variety of tools and approaches are needed to improve draft genomes.Results We used a custom assembly workflow to optimize consensus genome map assembly, resulting in an assembly equal to the estimated length of the Tribolium castaneum genome and with an N50 of more than 1 Mb. We used this map for super scaffolding the T. castaneum sequence assembly, more than tripling its N50 with the program Stitch.Conclusions In this article we present software that leverages consensus genome maps assembled from extremely long single molecule maps to increase the contiguity of sequence assemblies. We report the results of applying these tools to validate and improve a 7x Sanger draft of the T. castaneum genome.