RT Journal Article SR Electronic T1 A single chromosome assembly of Bacteroides fragilis strain BE1 from Illumina and MinION nanopore sequencing data JF bioRxiv FD Cold Spring Harbor Laboratory SP 024323 DO 10.1101/024323 A1 Judith Risse A1 Marian Thomson A1 Garry Blakely A1 Georgios Koutsovoulos A1 Mark Blaxter A1 Mick Watson YR 2015 UL http://biorxiv.org/content/early/2015/08/11/024323.abstract AB Background Second and third generation sequencing technologies have revolutionised bacterial genomics. Short-read Illumina reads result in cheap but fragmented assemblies, whereas longer reads are more expensive but result in more complete genomes. The Oxford Nanopore MinION device is a revolutionary mobile sequencer that can produce thousands of long, single molecule reads.Results We sequenced Bacteroides fragilis strain BE1 using both the Illumina MiSeq and Oxford Nanopore MinION platforms. We were able to assemble a single chromosome of 5.18 Mb, with no gaps, using publicly available software and commodity computing hardware. We identified gene rearrangements and the state of invertible promoters in the strain.Conclusions The single chromosome assembly of Bacteroides fragilis strain BE1 was achieved using only modest amounts of data, publicly available software and commodity computing hardware. This combination of technologies offers the possibility of ultra-cheap, high quality, finished bacterial genomes.