TY - JOUR T1 - A single chromosome assembly of <em>Bacteroides fragilis</em> strain BE1 from Illumina and MinION nanopore sequencing data JF - bioRxiv DO - 10.1101/024323 SP - 024323 AU - Judith Risse AU - Marian Thomson AU - Garry Blakely AU - Georgios Koutsovoulos AU - Mark Blaxter AU - Mick Watson Y1 - 2015/01/01 UR - http://biorxiv.org/content/early/2015/08/11/024323.abstract N2 - Background Second and third generation sequencing technologies have revolutionised bacterial genomics. Short-read Illumina reads result in cheap but fragmented assemblies, whereas longer reads are more expensive but result in more complete genomes. The Oxford Nanopore MinION device is a revolutionary mobile sequencer that can produce thousands of long, single molecule reads.Results We sequenced Bacteroides fragilis strain BE1 using both the Illumina MiSeq and Oxford Nanopore MinION platforms. We were able to assemble a single chromosome of 5.18 Mb, with no gaps, using publicly available software and commodity computing hardware. We identified gene rearrangements and the state of invertible promoters in the strain.Conclusions The single chromosome assembly of Bacteroides fragilis strain BE1 was achieved using only modest amounts of data, publicly available software and commodity computing hardware. This combination of technologies offers the possibility of ultra-cheap, high quality, finished bacterial genomes. ER -