RT Journal Article
SR Electronic
T1 DADA2: High resolution sample inference from amplicon data
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 024034
DO 10.1101/024034
A1 Benjamin J Callahan
A1 Paul J McMurdie
A1 Michael J Rosen
A1 Andrew W Han
A1 Amy Jo Johnson
A1 Susan P Holmes
YR 2015
UL http://biorxiv.org/content/early/2015/08/06/024034.abstract
AB Microbial communities are commonly characterized by amplifying and sequencing target genes, but errors limit the precision of amplicon sequencing. We present DADA2, a software package that models and corrects amplicon errors. DADA2 identified more real variants than other methods in Illumina-sequenced mock communities, some differing by a single nucleotide, while outputting fewer spurious sequences. DADA2 analysis of vaginal samples revealed a diversity of Lactobacillus crispatus strains undetected by OTU methods.