RT Journal Article
SR Electronic
T1 Genomic DNA transposition induced by human PGBD5
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 023887
DO 10.1101/023887
A1 Anton G. Henssen
A1 Elizabeth Henaff
A1 Eileen Jiang
A1 Amy R. Eisenberg
A1 Julianne R. Carson
A1 Camila M. Villasante
A1 Mondira Ray
A1 Eric Still
A1 Melissa Burns
A1 Jorge Gandara
A1 Cedric Feschotte
A1 Christopher E. Mason
A1 Alex Kentsis
YR 2015
UL http://biorxiv.org/content/early/2015/08/03/023887.abstract
AB Transposons are mobile genetic elements that are found in nearly all organisms, including humans. Mobilization of DNA transposons by transposase enzymes can cause genomic rearrangements, but our knowledge of human genes derived from transposases is limited. Here, we find that the protein encoded by human PGBD5, the most evolutionarily conserved transposable element-derived gene in chordates, can induce stereotypical cut-and-paste DNA transposition in human cells. Genomic integration activity of PGBD5 requires distinct aspartic acid residues in its transposase domain, and specific DNA sequences with inverted terminal repeats with similarity to piggyBac transposons. DNA transposition catalyzed by PGBD5 in human cells occurs genome-wide, with precise transposon excision and preference for insertion at TTAA sites. The apparent conservation of DNA transposition activity by PGBD5 raises the possibility that genomic remodeling may contribute to its biological function.