@article {Boyce023796, author = {Sarah E. Boyce and Joel Tellinghuisen and John D. Chodera}, title = {Avoiding accuracy-limiting pitfalls in the study of protein-ligand interactions with isothermal titration calorimetry}, elocation-id = {023796}, year = {2015}, doi = {10.1101/023796}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Isothermal titration calorimetry (ITC) can yield precise (3\%) estimates of the thermodynamic parameters describing biomolecular association (affinity, enthalpy, and entropy), making it an indispensable tool for biochemistry and drug discovery. Surprisingly, interlaboratory comparisons suggest that errors of ∽ 20\% are common and widely underreported. Here, we show how to reduce precision- and accuracy-limiting errors while obtaining good estimates and minimizing material and time consumed by an experiment. We provide a simple spreadsheet that allows practitioners to identify precision-limiting operations during protocol design, track precision during the experiment, and propagate error to yield realistic final uncertainties.}, URL = {https://www.biorxiv.org/content/early/2015/08/03/023796}, eprint = {https://www.biorxiv.org/content/early/2015/08/03/023796.full.pdf}, journal = {bioRxiv} }