PT  - JOURNAL ARTICLE
AU  - Bastiaan Spanjaard
AU  - Bo Hu
AU  - Nina Mitic
AU  - Jan Philipp Junker
TI  - Massively parallel single cell lineage tracing using CRISPR/Cas9 induced genetic scars
AID  - 10.1101/205971
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 205971
4099  - http://biorxiv.org/content/early/2017/10/19/205971.short
4100  - http://biorxiv.org/content/early/2017/10/19/205971.full
AB  - A key goal of developmental biology is to understand how a single cell transforms into a full-grown organism consisting of many different cell types. Single-cell RNA-sequencing (scRNA-seq) has become a widely-used method due to its ability to identify all cell types in a tissue or organ in a systematic manner 1–3. However, a major challenge is to organize the resulting taxonomy of cell types into lineage trees revealing the developmental origin of cells. Here, we present a strategy for simultaneous lineage tracing and transcriptome profiling in thousands of single cells. By combining scRNA-seq with computational analysis of lineage barcodes generated by genome editing of transgenic reporter genes, we reconstruct developmental lineage trees in zebrafish larvae and adult fish. In future analyses, LINNAEUS (LINeage tracing by Nuclease-Activated Editing of Ubiquitous Sequences) can be used as a systematic approach for identifying the lineage origin of novel cell types, or of known cell types under different conditions.